Mass Spectrometry Facility Useful Information

Last Updated:  8/4/2008

Sample Preparation Reference Documents

• MSF Standard Operation Procedures (read this first)
• Gel Band Preparation/Trypsin Digestion Procedures
• MALDI sample preparation for DNA
• Short guidelines for MALDI sample preparation
• Making MALDI spots with CCA matrix
• Making MALDI spots with FA matrix
• Making MALDI spots with SA matrix

Training Documents

• Introduction to Walk-Up Mass Spectrometry (7/21/08)  (pdf or PowerPoint)
• Analyzing Small Molecules by ESI-TOF (7/28/08)  (pdf or PowerPoint)
• Introduction to Organic Mass Spectrometry (9/10/07 and 9/12/07)
• Biflex III MALDI-TOF User Training (7/29/08)
  • Intro slides (pdf)
  • Sofware Screenshots (pdf)
  • Full PowerPoint (PowerPoint)
• Analyzing Semi-Volatiles by GC-MS (7/31/08) (pdf or PowerPoint)
• Analysing Large Molecules by ESI-TOF MS (8/4/08) (pdf or PowerPoint)
• How to access GC-MS data from outside the MSF and ChemStation Tutorial
• Quick MALDI sample preparation for proteins

Calibration Reference Documents

• Common positive ion protein/petide calibrants used with the Bruker Biflex III MALDI-TOF
• Perfluorokerosene masses (MAT 95 calibrants)
• Common MALDI-TOF calibration mixtures

 Other Useful Info

• Glossary of Mass Spectrometry Terms
• Table of Isotopic Compositions and Atomic Weights (NIST)
• Mass Spectrometry-related constants and equations
• Some Protein MS and MS-MS tutorial websites

• http://www.ionsource.com/tutorial/spectut/spec1.htm
• http://www.chm.bris.ac.uk/ms/theory/esi-ionisation.html
• http://www.newobjective.com/electrospray/
• http://staging.mc.vanderbilt.edu/msrc/tutorials/ms/ms.htm
• http://www.ionsource.com/Card/aatable/aatable.htm
• http://www.ionsource.com
• http://www.cas.astate.edu/geochemistry/mass%20spec/electrospray%20MS.pdf
• http://www.proteomesoftware.com/Proteome_software_Education.html
• http://www.abrf.org/ResearchGroups/MassSpectrometry/EPosters/ms97quiz/SequencingTutorial.htm
• http://www.ionsource.com/tutorial/protID/idtoc.htm